Assuming we already know the following parameters of our image condition on a TEM: Voltage=300 Pixel size = 1 Å Dose rate = 8 (electron/pixel/second) Exposure time = 10 s Number of frames per movie= 40 How to calculate total dose per movie? Total dose (electron/Å^2)= Dose rate (electron/Å^2/second) x exposure time (s) = 8 (electron/pixel/second)/ (1 Å x 1 Å ) x 10 s = 80 (electron/Å^2) How to calculate dose per frame? Dose per frame (electron/Å^2)= Total dose (electron/Å^2) / number of frames = 80 (electron/Å^2) / 40 = 2 (electron/Å^2)