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How to calculate total dose and dose per frame with a given image condition?

Assuming we already know the following parameters of our image condition on a TEM:

Voltage=300
Pixel size = 1 Å
Dose rate = 8 (electron/pixel/second)
Exposure time = 10 s
Number of frames per movie= 40

How to calculate total dose per movie?


Total dose (electron/Å^2)= Dose rate (electron/Å^2/second) x exposure time (s)
                                       = 8 (electron/pixel/second)/ (1 Å x 1 Å ) x 10 s
                                       = 80 (electron/Å^2)

How to calculate dose per frame?


Dose per frame (electron/Å^2)= Total dose (electron/Å^2) / number of frames
                                               = 80 (electron/Å^2) / 40
                                               = 2 (electron/Å^2)

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